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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -BRCA1, phospyhorylated (Ser1423) (Breast Cancer 1, Early Onset, Breast-Ovarian Cancer Susceptibility)

Anti -BRCA1, phospyhorylated (Ser1423) (Breast Cancer 1, Early Onset, Breast-Ovarian
Cancer Susceptibility)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #B2709-01D1
ApplicationsSuitable for use in Western Blot and Peptide Inhibition. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 1-2ug/ml of this lot detected phospho-BRCA1 (Ser1423) in HeLa nuclear extract.
Peptide Inhibition Analysis: 5uM of the immunizing peptide abolished detection of phospho-BRCA1 (Ser1423) in immunoblot analysis of HeLa nuclear extract.
Optimal dilutions to be determined by the researcher.
Included Positive Antigen ControlHeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide containing the sequence ...GpSQP... in which pS corresponds to phosphoserine 1423 of human BRCA1.
SpecificityRecognizes human phospho-BRCA1 (Ser1423), Mr 200-220kD. Specificity was demonstrated by blocking the antibody with the immunizing peptide and treating the blot with lambda-phosphatase.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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