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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -BRCA1 (Breast Cancer Antigen, Early Onset Breast Ovarian Cancer Susceptibility Protein 1)

Anti -BRCA1 (Breast Cancer Antigen, Early Onset Breast Ovarian Cancer Susceptibility
Protein 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IP IH
Catalog #B2709-03
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 1:5000-1:10,000 dilution of this lot detected BRCA1 in HeLa nuclear extract.
Immunohistochemistry: This antibody has been reported by an independent laboratory to detect BRCA1 in breast cancer tumor sections.
Immunoprecipitation: This antibody has been reported by an independent laboratory to immunoprecipitate BRCA1.
Optimal dilutions to be determined by the researcher.
Included Positive Antigen ControlHeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid containing 0.05% sodium azide before the addition of glycerol to 30%.
PuritySerum
ImmunogenGST-tagged fusion protein corresponding to aa1301-1863 of human BRCA1
SpecificityRecognizes human BRCA1, Mr ~220kD. A non-specific protein was also detected at Mr ~50kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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