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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -BRCA1, phosphorylated (Ser1497) (Breast Cancer Antigen, Early Onset Breast Ovarian Cancer Susceptibility Protein 1)

Anti -BRCA1, phosphorylated (Ser1497) (Breast Cancer Antigen, Early Onset Breast Ovarian
Cancer Susceptibility Protein 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #B2727
Applications Suitable for use in ELISA and Immunoblot. Other applications not tested.
Recommended Dilution Immunoblot Analysis: 1ug/ml detects phosphorylated BRCA1 in HeLa Nuclear Extract. HeLa nuclear extract was cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-BRCA1 (1ug/ml). Proteins were visualized
using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence
detection system.
Optimal dilutions to be determined by the researcher.
Included Positive Antigen Control HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10ul of extract and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
Form0.1M Tris glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of 30% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenKLH-conjugated, synthetic phosphopeptide corresponding to residues 1489-1500 of human BRCA1 (C-KEPGVERS[pS]PSK).
SpecificityRecognizes phosphorylated BRCA1, Mr 250kD. A non-specific doublet also detected at 95kD in HeLa nuclear extract. Specificity was demonstrated by blocking the antibody with the immunizing peptide or treating the lysate with lambda-phosphatase.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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