Anti -BRCC-36 (BRCA1/BRCA2-containing Complex Subunit 36, BRCC36, BRCA1/BRCA2-containing
Complex Subunit 3, BRCC3, C6.1A, CXorf53, RP11-143H17.2)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP FC |
|
| BRCC-36 is a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. It is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains, and does not have activity toward 'Lys-48'-linked polyubiquitin chains. In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint (1). | | | Catalog # | B2727-01D | | Applications | Suitable for use in Flow Cytometry, Western Blot, and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1:100-1:500 | | Western Blot: 1:1000-1:50,000 | | Immunoprecipitation: 1:10-1:100 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Clone No | 10J91 | | Host | Rabbit | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol. | | Purity | Supernatant | | Immunogen | Synthetic peptide corresponding to residues in human BRCC-36. | | Specificity | Recognizes human BRCC-36 at ~36kD. Species Crossreactivity: mouse, rat | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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