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You are here:Home » Antibodies » Abs to Disease Markers » Anti -Breast Cancer Resistance Protein (BCRP)

Anti -Breast Cancer Resistance Protein (BCRP)

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Specifications

Clone Host Grade Applications
Monoclonal Rat B IH IC FC
An ABC transporter. Allows efflux of Hoechst dye, a property that has been used to separate bone marrow side population cells, which express BCRP/ABCG2. Appears to play a major role in the multidrug resistance phenotype of a specific MCF-7 breast cancer cell line. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
Catalog #B2738-02A
Cellular Localization Plasma membrane, integral membrane protein
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:20-1:50
Immunohistochemistry: 1:20
Immunocytochemistry: 1:20-1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8J379
HostRat
SourceMouse
Concentration~0.25mg/ml
Form0.7% BSA, tissue culture supernatant (serum free), 0.1% Sodium Azide
PurityTissue culture supernatant
ImmunogenSynthetic peptide, corresponding to amino acids 221-394 of mouse BCRP/ABCG2 fused to maltose binding protein.
SpecificityThe BXP-9 Mab was selected after immunization with a fusion protein containing the E. coli maltose binding protein and a fragment of the mouse bcrp protein corresponding to amino acids 221-394. BXP-9 reacts with an internal epitope of bcrp, a 70kD transmembrane half-transporter which is involved in Multidrug resistance. BXP-9 does not react with the human BCRP molecule. Species Crossreactivity: This antibody reacts with mouse. It does not react with human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesABCG2


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