| An ABC transporter. Allows efflux of Hoechst dye, a property that has been used to separate bone marrow side population cells, which express BCRP/ABCG2. Appears to play a major role in the multidrug resistance phenotype of a specific MCF-7 breast cancer cell line. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123. |
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| Catalog # | B2738-02A |
| Cellular Localization | Plasma membrane, integral membrane protein |
| Applications | Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:20-1:50 |
| Immunohistochemistry: 1:20 |
| Immunocytochemistry: 1:20-1:50 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 8J379 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~0.25mg/ml |
| Form | 0.7% BSA, tissue culture supernatant (serum free), 0.1% Sodium Azide |
| Purity | Tissue culture supernatant |
| Immunogen | Synthetic peptide, corresponding to amino acids 221-394 of mouse BCRP/ABCG2 fused to maltose binding protein. |
| Specificity | The BXP-9 Mab was selected after immunization with a fusion protein containing the E. coli maltose binding protein and a fragment of the mouse bcrp protein corresponding to amino acids 221-394. BXP-9 reacts with an internal epitope of bcrp, a 70kD transmembrane half-transporter which is involved in Multidrug resistance. BXP-9 does not react with the human BCRP molecule. Species Crossreactivity: This antibody reacts with mouse. It does not react with human. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | ABCG2 |
