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You are here:Home » Antibodies » Antibodies-Disease Markers » Anti -Breast Cancer Resistance Protein (BCRP)

Anti -Breast Cancer Resistance Protein (BCRP)


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Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH IC
Breast Cancer Resistance Protein (BCRP) is a 70kD ATP-Binding Cassette membrane transport protein involved in multidrug resistance. BCRP may be over-expressed in cancer cell lines selected with doxorubicin / verapamil, topotecan or mitoxantrone.
Catalog #B2738A
Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionsImmunohistochemistry (Frozen): 1:20 dilution on acetone fixed frozen sections followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex.
Immunohistochemistry (Paraffin): Use on formaldehyde-fixed paraffin-embedded human tissues and tumors after pretreatment with 10mM citric acid, pH 6.0, for 20 min.
Immunocytochemistry: 1:20-1:50 on acetone fixed cytospin preparations.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No6D171
ConcentrationNot determined
FormSupplied as a liquid in 0.7% BSA, 0.1% sodium azide.
ImmunogenRecombinant protein corresponding to aa271-396 from human Breast Cancer Resistance Protein.
SpecificityRecognizes an internal epitope of human Breast Cancer Resistance Protein. Does not cross-react with human P-glycoprotein, MRP1 or MRP2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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