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You are here:Home » Antibodies » Abs to Carbohydrates, Glycoproteins » Anti -Brevican (BEHAB, Brain-Enriched Hyaluronan-Binding Protein)

Anti -Brevican (BEHAB, Brain-Enriched Hyaluronan-Binding Protein)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B
Brevican (brain-enriched hyaluronan-binding protein) is a 160kD member of the aggrecan/versican proteoglycan family of matrix proteins. It is brain-derived and serves as a linker between HA and other matrix molecules such as tenascins and fibulins. Mature human Brevican is 889 amino acids in length. It contains multiple domains, with an N-terminal V-type Ig-like domain (aa # 52-139) and a C-terminal EGF-like domain (aa # 646-682) and a C-type lectin domain (aa # 695-802). Multiple isoforms exist where there is an alternate start site at Met228 and a 23 aa substitution for aa # 649-911 that serves as a GPI-anchor motif. Over aa #23-911, human Brevican shares 82% aa identity with mouse Brevican.
Catalog #B2739-70
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.2ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
PurityPurified by human Brevican affinity chromatography.
ImmunogenNS0-derived recombinant human Brevican isoform 1 (aa23-911) Accession # Q96GW7, Lys504Glu substitution
SpecificityRecognizes human Brevican.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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