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You are here:Home » Antibodies » Abs to Enzymes, Polymerase » Anti -BRF2 (BRFU, FLJ11052, RNA Polymerase III Transcription Initiation Factor BRF2, TFIIIB50, Transcription Factor IIB-related Factor TFIIIB50)

Anti -BRF2 (BRFU, FLJ11052, RNA Polymerase III Transcription Initiation Factor BRF2,
TFIIIB50, Transcription Factor IIB-related Factor TFIIIB50)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
BRF2 is one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. BRF2 is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein.
Catalog #B2743-53
Cellular Localization Nuclear
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:16,000.
Western Blot: 1-3ug/ml. Predicted molecular weight 48kD. Detects a band of ~ 50kD
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in TBS, pH 7.2, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide: QAARQAATSVPNPP, representing the to C- terminus of the human protein (residues 406-419), according to NP_060780.
SpecificitySpecies Crossreactivity: Crossreacts with Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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