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You are here:Home » Culture Media » Media-Microbiological » Brilliant Green Agar w/Sulfadiazine (Powder)

Brilliant Green Agar w/Sulfadiazine (Powder)

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Specifications

Selective isolation of Salmonella from food, especially egg products.
Catalog #B2750-02
DescriptionBrilliant Green Agar was first described by Kristensen et al. in 1925 and later modified by Kauffman in 1933. It is classified by the USP as a highly selective medium for the recovery of Salmonella species except for tyhphoid and paratyphoid strains. Brilliant Green dye inhibits Gram positive and most Gram negative bacteria. Phenol red serves as a pH indicator for the fermentation of lactose or sucrose in the medium. Osborne and Stokes used 0.1% Sodium Sulfapyridine to enhance the recovery of Salmonella from whole egg and egg yolk. The use of sulfonamides further inhibits Escherichia coli and Proteus sp.
StorageStore the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light greenish beige color.
Microbiological Analysis
Enterococcus faecalis ATCC 29212Inhibited
Escherichia coli ATCC 25922Slightly Inhibited Yellow
Salmonella enteritidis ATCC 13076Growth Pink-white
Salmonella typhi ATCC 19430Slightly Inhibited Red
Salmonella typhimurium ATCC 14028Growth Pink-white
Appearance
Light beige with a greenish tint, homogeneous, free flowing powder.
Solubility
Brownish-green, slightty turbid.
pH (25°C)
6.9±0.2
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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