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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -Brk

Anti -Brk

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #B2765-01
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500 dilution detected endogenous Brk in SKBR3 cell lysate. Lysate was resolved by electrophoresis, transferred to PVDF, and probed with B2765-01 for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Band observed at ~50kD indicates Brk. An upper band is thought to be the 73kD ELF-related protein that shares 70% homology with the immunizing peptide.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~0.5mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, 0.15M sodium chloride, pH 7.4, containing 0.05% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to the N-terminal region of human Brk.
SpecificityRecognizes Brk (breast tumor kinase, PTK6) at approximately 52 kDa. It can also recognize the 73 kDa ELF-related protein that shares 70% homology with the immunizing peptide.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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