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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IP IH FC

Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated, disulfide-linked homodimer. Recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.1-2
Catalog #	B2788-45B
Applications	• Flow cytometry 2
	• Immunoprecipitation 2
	• Immunohistochemistry (acetone-fixed, frozen tissue sections)
Recommended Dilution	Flow Cytometry: 1ug/10e6 cells
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	5K98
Host	Mouse
Source	Chicken
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
Purity	Purified
Immunogen	Bursal cells from one-day-old H.B15 (Bu-1a/b) strain chickens
Specificity	Recognizes chicken Bu-1 (chB6), Mr 70-75kD. Does not crossreact with turkey cells when analyzed by immunofluorescent staining and flow cytometry.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.