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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH FC

Bu-1a is a 70kD alloantigen expressed on B cells and a subset of macrophages. Reports suggest that Bu-1 is expressed on B cell precursors at early stages of development.
Catalog #	B2788-50D
Applications	Suitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested.
Recommended Dilution	Flow Cytometry: 1:200 -1:1,000; 10ul labels 10e6 cells in 100ul
	Optimal dilutions to be determined by the researcher.
Hybridoma	SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	10B337
Host	Mouse
Source	Chicken
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Chicken Bursa cells.
Specificity	Recognizes the 'a' allele of the polymorphic cell surface antigen Bu-1.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.