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You are here:Home » Antibodies » Abs to Cell Markers » Anti -Bu-1b (FITC)

Anti -Bu-1b (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IP IH FC
Chicken Bu-1b, a product of the Bu-1b allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion.
Catalog #B2788-55A
Applications Flow cytometry 1
Immunoprecipitation 1
Immunohistochemistry (frozen sections)
Recommended DilutionFlow Cytometry: 1ug/10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5K94
HostMouse
SourceChicken
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide. Conjugated to FITC.
PurityPurified
ImmunogenBursal cells from 2- to 5-week-old CHA (Bu-1b) strain chickens
SpecificityRecognizes chicken Bu-1b alloantigen, Mr 70kD. Does not react with cells from CHA and H.B15Ab strains by immunofluorescence.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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