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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -BubR1 (Bub1b, Bub1A, MAD3L, budding uninhibited by benzimidazoles related 1, budding uninhibited by benzimidazoles 1 homolog beta, mitotic arrest deficient-like 3 like)

Anti -BubR1 (Bub1b, Bub1A, MAD3L, budding uninhibited by benzimidazoles related 1, budding
uninhibited by benzimidazoles 1 homolog beta, mitotic arrest deficient-like 3 like)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
Catalog #B2790-40
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:2500-1:7500. Whole cell lysate (50ug for Input, 1mg for IP) from HeLa cells that were untreated (-, interphase) or treated with Nocodazole (+, mitotic). Antibody used at 0.2ug/ml for Western Blot and at 3ug/mg lysate for IP.
Immunoprecipitation: 1-5ug/mg lysate. Immunoprecipitated using another affinity purified rabbit anti-BubR1 antibody to a different epitope.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.1% sodium azide, before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenRegion between residues 350 and 400 of human budding uninhibited by benzimidazoles 1 homolog beta using the numbering given in TrEMBL entry Q8WV50 (GeneID 701).
SpecificityRecognizes human BubR1. Species sequence homology: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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