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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -BubR1 (Bub1b, Bub1A, MAD3L, budding uninhibited by benzimidazoles related 1, budding uninhibited by benzimidazoles 1 homolog beta, mitotic arrest deficient-like 3 like)

Anti -BubR1 (Bub1b, Bub1A, MAD3L, budding uninhibited by benzimidazoles related 1, budding
uninhibited by benzimidazoles 1 homolog beta, mitotic arrest deficient-like 3 like)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IF
Compromised spindle checkpoints are thought to play an important role in genetic instability that predisposes cells to malignant transformations. Human cells contain two related checkpoint protein kinases, evolved from the BUB1 gene; hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotic kinetochore-microtubule interactions. hBUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. It has been identified as a novel binding partner of BCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate the progression of breast cancer by compromising the mitotic checkpoint control through inactivation of BUBR1.
Catalog #B2790-40B
Cellular Localization Nuclear
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No8J382
HostMouse
SourceHuman
Concentration~1mg/ml
FormAs reported
PurityPurified by Protein G affinity chromatography.
ImmunogenA portion of hBUBR1 tagged at the N-terminus with pMAL (residues 1-350 of human BUBR1).
SpecificitySpecific for hBUBR1. There should be no crossreactivity with hBUB1. Species Crossreactivity: Human and mouse. Other species have not been tested.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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