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You are here:Home » Antibodies » Abs to DNA Replication, Repair » Anti -BubR1 (BUB1beta, BUB1B, Bub1A, hBUBR1, MAD3L, MAD3/BUB1-related Protein Kinase, Mitotic Checkpoint Kinase MAD3L, Mitotic Checkpoint Serine/threonine-protein Kinase BUB1 beta, Protein SSK1)

Anti -BubR1 (BUB1beta, BUB1B, Bub1A, hBUBR1, MAD3L, MAD3/BUB1-related Protein Kinase,
Mitotic Checkpoint Kinase MAD3L, Mitotic Checkpoint Serine/threonine-protein Kinase BUB1
beta, Protein SSK1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified B
The Mitotic Checkpoint Complex (MCC) , which contains Bub1, Bub1b, Bub3, Mad2 and Cdc20, controls chromosome segregation and monitors kinetochoremicrotubule interactions (1). This complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/ Cyclosome (APC/C) during mitosis to prevent cells with unaligned chromosomes from prematurely entering
anaphase (2). Bub1b and Bub1 kinases are mutated in several types of human malignancies including hematopoietic, colorectal, lung, and breast cancers (3). Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome (4). Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes and increased cancer susceptibility (5). Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores (6) and is required for functional microtubule-kinetochore interactions (7).
Catalog #B2790-42
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No11C1018
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
PurityPurified
ImmunogenSynthetic peptide corresponding to aa sequence surrounding Lys370 of human Bub1b.
SpecificityRecognizes endogenous levels of total Bub1b.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesBudding Uninhibited by Benzimidazoles 1 Homolog beta (Yeast), EC=2.7.11.1


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