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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified E B IP IH IF

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Catalog #	B2850-01
Applications	Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Fluorescent In Situ Hybridization (FISH) Assay. Other applications not tested.
Recommended Dilutions	Western Blot: Band is observed at ~307kD
	Immunohistochemistry: Frozen and paraffin sections. Enzymatic antigen retrieval and DNA acid denaturation required.
	Fluorescent In Situ Hybridization: 1:25; Use a rabbit anti-sheep AMCA secondary antibody.
	Note: Denature DNA sample first so base is accessible to BrdU antibody.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	N/A
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.6. No preservative added.
Purity	Purified by Protein G affinity chromatography followed by polishing chromatography against resin coupled to HGG.
Immunogen	Bromodeoxyuridine, conjugated to HGG (Human Gamma Globulin)
Specificity	Recognizes Bromodeoxyuridine at ~307kD. Species Crossreactivity: all species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.