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You are here:Home » Antibodies » Abs to Nucleotides » Anti -Bromodeoxyuridine (BrdU)

Anti -Bromodeoxyuridine (BrdU)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IH FC
BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Catalog #B2850-01B
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry.
Other applications have not been tested.
Recommended DilutionFlow Cytometry: 1:25-1:200. Use 20ul to label 10e6 cells in 100ul
Immunohistochemistry (Paraffin): 1:25-1:200
Optimal working dilutions to be determined by researcher.
Recommended Secondary ReagentsI1904-49H IgG, F(abí)2, H&L X-Adsorbed (HRP) RbxRt
I1904-49U IgG, F(abí)2, H&L X-Adsorbed (FITC) RbxRt
Recommended Negative ControlsI1904-83M1: IgG2a, Rat Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H579
HostRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenBromodeoxyuridine (BrdU)
SpecificityRecognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Does not crossreact with thymidine but does react weakly with chlorodeoxyuridine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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