| BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors. |
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| Catalog # | B2850-01B |
| Applications | Suitable for use in Flow Cytometry and Immunohistochemistry. |
| Other applications have not been tested. |
| Recommended Dilution | Flow Cytometry: 1:25-1:200. Use 20ul to label 10e6 cells in 100ul |
| Immunohistochemistry (Paraffin): 1:25-1:200 |
| Optimal working dilutions to be determined by researcher. |
| Recommended Secondary Reagents | I1904-49H IgG, F(ab’)2, H&L X-Adsorbed (HRP) RbxRt |
| I1904-49U IgG, F(ab’)2, H&L X-Adsorbed (FITC) RbxRt |
| Recommended Negative Controls | I1904-83M1: IgG2a, Rat Negative Control |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 3H579 |
| Host | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Bromodeoxyuridine (BrdU) |
| Specificity | Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Does not crossreact with thymidine but does react weakly with chlorodeoxyuridine. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
