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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH IC FC

The life cycle of a eukaryotic cell consists of various phases, two of which can easily be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques. In between the M- and S-phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a ‘quiescent’ phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S and from G2 to M-phase.
Catalog #	B2850-01F
Applications	Suitable for use in Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilution	Flow Cyotmetry: 1:50-1:100
	Immunohistochemistry (Frozen and paraffin sections): Detection antibody with avidin-biotinylated horseradish peroxidase complex.
	Optimal dilutions to be determined by researcher.
Hybridoma	Hybridization of myeloma cells from SP2/0-Ag14 mice with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	4A9
Host	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of 40% glycerol. No stabilizing proteins added.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Bromodeoxyuridine (BrdU)-conjugate to BSA.
Specificity	Bromodeoxuridine, also when incorporated into nuclear DNA.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.