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You are here:Home » Antibodies » Abs to Nucleotides » Anti -Bromodeoxyuridine (BrdU)

Anti -Bromodeoxyuridine (BrdU)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IP IH
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Catalog #B2850-01G
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG2b
HostSheep
Concentration~1mg/ml
FormSupplied as a liquid in PBS. No preservative added.
PurityPurified by Protein G affinity chromatography.
ImmunogenHighly purified Bromodeoxyuridine, conjugated to KLH.
SpecificityRecognizes bromodeoxyuridine at ~307kD. Very low crossreactivity to 5-methyl cytosine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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