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You are here:Home » Antibodies » Abs to Nucleotides » Anti -Bromodeoxyuridine (BrdU)

Anti -Bromodeoxyuridine (BrdU)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse IH IC FC IF
BrdU is a thymidine analog that, when added to replicateing cells, is incorporated into newly synthesized DNA strands. Evaluating the uptake of BrdU by dividing cells can provide a reliable estimation of cellular prolieration rates. BrdU immunohistochemistry may prove useful in determining proliferative rates for the evaluation of tumors.
Catalog #B2850-01K
Positive Control Bromodeoxyuridine labeled cells.
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry (Fr) (paraffin-embedded sections) and Immunocytochemistry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8J385
HostMouse
ConcentrationNot determined
FormPBS with 1% BSA containing 0.09% sodium azide
PurityAscites
ImmunogenBrdU conjugated to BSA.
SpecificityThe antibody is specific for DNA in which BrdU has been incorporated. In immunoassays this antibody reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. In immuncytochemistry the antibody only recognizes BrdU in denaturated (single stranded) DNA. The BrdU antibody is 100% crossreactive with Iodo-Deoxy-Uridine (IrdU). Therfore, IdU instead of BrdU can be used in studies. Species Crossreactivity: Crossreacts with human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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