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You are here:Home » Antibodies » Abs to Nucleotides » Anti -Bromodeoxyuridine (BrdU) (HRP)

Anti -Bromodeoxyuridine (BrdU) (HRP)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E IH
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Catalog #B2850-01R
ApplicationsSuitable for use in ELISA and Immunohistochemistry (paraffin). Other applications not tested.
Recommended DilutionELISA: 1:250-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H579
HostRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH7.4, 0.01% thiomersal. Conjugated to HRP.
PurityPurified by Protein G affinity chromatography.
ImmunogenBromodeoxyuridine (BrdU).
SpecificityRecognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. It does not cross react with thymidine but does react weakly with chlorodeoxyuridine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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