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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified FC

BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Catalog #	B2850-01U
Applications	Suitable for use in Flow cytometry. Other applications have not been tested.
Recommended Dilutions
	Flow cytometry: Neat. Use 20ul to label 10e6 cells in 100ul.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	BU1/75 (ICR1)
Host	Rat
Concentration	~0.1mg/ml
Form	Suppplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Bromodeoxyuridine (BrdU)
Specificity	Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Does not cross react with thymidine but does react weakly with chlorodeoxyuridine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	5-Bromodeoxyuridine