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You are here:Home » Antibodies » Abs to Nucleotides » Anti -Bromodeoxyuridine (BrdU) (FITC)

Anti -Bromodeoxyuridine (BrdU) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Highly Purified IH FC
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Catalog #B2850-02D
ApplicationsSuitable for use in Flow Cytometry, Immunohistology. Other applications not tested.
Recommended DilutionFlow Cytometry: Neat 20ul labels 10e6 cells in 100ul.
Immunohistology: Neat 1/20
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7E101
HostRat
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.01% sodium azide, before the addition of 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).
PurityPurified by Ion Exchange chromatography.
ImmunogenBrdU
SpecificityRecognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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