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You are here:Home » Antibodies » Abs to Nucleotides » Anti -Bromodeoxyuridine (BrdU)

Anti -Bromodeoxyuridine (BrdU)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IF
Catalog #B2850-03
ApplicationsSuitable for use in Immunofluorescence microscopy. Also suitable for Detection of bromodeoxyuridine incorporation in tissue and cell culture; 3H-thymidine labeling techniques can be avoided.
Recommended DilutionImmunofluorescence microscopy: 1:10; Incubation Time 30 min at RT.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5F172
HostMouse
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to a final volume of 1ml.
PurityPurified by Protein A affinity chromatography.
ImmunogenBromodeoxyuridine.
SpecificitySpecific for bromodesoxyuridine and does not crossreact with thymidine. It is a valuable tool for detection of S-phase (DNA-synthesizing cells) in cell and tissue culture.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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