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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IF

Catalog #	B2850-03
Applications	Suitable for use in Immunofluorescence microscopy. Also suitable for Detection of bromodeoxyuridine incorporation in tissue and cell culture; 3H-thymidine labeling techniques can be avoided.
Recommended Dilution	Immunofluorescence microscopy: 1:10; Incubation Time 30 min at RT.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	5F172
Host	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to a final volume of 1ml.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Bromodeoxyuridine.
Specificity	Specific for bromodesoxyuridine and does not crossreact with thymidine. It is a valuable tool for detection of S-phase (DNA-synthesizing cells) in cell and tissue culture.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.