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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites IH FC

Catalog #	B2850-03A
Applications	Suitable for use in In situ-hybridization, Flow Cytometry and Immunohistochemistry (Frozen and paraffin sections)
Recommended Dilution	Immunohistochemistry: 1:10; Incubation Time 1 hr at room temperature.
It can be used for	1. Radioimmunocytochemical detection of circulating levels of BrdU
	2 Detection of S-phase cells in tissue sections by immunoperoxidase or immunofluorescence method
	3. Detection of S-phase cells in cell suspension
	4. Determination of the percentage of profilerating cells by flow cytometry
	5. Quantitative determination of the number of various phases of the cell cycle by dual parameter flow-cytometrical analysis
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F214
Host	Mouse
Concentration	Not determined
Form	Supplied as a liquid.
Purity	Ascites
Immunogen	BrdU conjugated to BSA
Specificity	Reacts with BrdU in denatured (single-stranded) DNA). The antibody is crossreactive with iododeoxyuridine. Species Crossreactivity: All species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.