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You are here:Home » Antibodies » Abs to Nucleotides » Anti -Bromodeoxyuridine (BrdU)

Anti -Bromodeoxyuridine (BrdU)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Purified E B IP IH
Catalog #B2850-04B
Application(s) Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, ELISA.
Recommended DilutionImmunoprecipitation 25-100ug/ml. Tested against 5-Methyl Cytosine (5-MeC) and bromo-deoxyuridine (BrdU) or control (no antigen) and assayed by A-405 spectrophotometry. At a concentration of 25ug/ml, this product demonstrates 8 fold higher reactivity with 5-MeC versus BrdU. Product titers out at 50ng/ml. For best results, use the product at 25-100ug/ml. Nearly complete immununoprecipitation was obtained at 100-500ug/ml.
Immunohistochemistry Frozen and Paraffin sections 10ug/ml. Stains bromodeoxyuridine incorporated into DNA of replicating cells. Denature DNA sample-first so base is accessable to BrdU antibody.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
ConcentrationAs reported
FormSupplied in PBS without azide.
PurityPurified
ImmunogenBromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH).
SpecificityRecognizes Bromodeoxyuridine (BrdU). At 25ug/ml, demonstrates 8 fold higher reactivity with 5-MeC versus BrdU.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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