Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Polyclonal Sheep Purified E B IP IH

Catalog #	B2850-04B
Application(s)	Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, ELISA.
Recommended Dilution	Immunoprecipitation 25-100ug/ml. Tested against 5-Methyl Cytosine (5-MeC) and bromo-deoxyuridine (BrdU) or control (no antigen) and assayed by A-405 spectrophotometry. At a concentration of 25ug/ml, this product demonstrates 8 fold higher reactivity with 5-MeC versus BrdU. Product titers out at 50ng/ml. For best results, use the product at 25-100ug/ml. Nearly complete immununoprecipitation was obtained at 100-500ug/ml.
Immunohistochemistry Frozen and Paraffin sections	10ug/ml. Stains bromodeoxyuridine incorporated into DNA of replicating cells. Denature DNA sample-first so base is accessable to BrdU antibody.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Concentration	As reported
Form	Supplied in PBS without azide.
Purity	Purified
Immunogen	Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH).
Specificity	Recognizes Bromodeoxyuridine (BrdU). At 25ug/ml, demonstrates 8 fold higher reactivity with 5-MeC versus BrdU.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.