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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH FC IF

BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Catalog #	B2850-07
Applications
	Suitable for use in Flow Cytometry, Immuno uorescence and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions	Immunohistochemistry (formalin/paraf n): 1:500 for 32 minutes at RT. For staining of formalin- xed tissues, incubate sections in 4N HCl for 30 minutes at RT, followed by digestion with trypsin at 1mg/ml PBS, 10 minutes at 37ºC .
	Optimal dilutions to be determined by the researcher.
Positive Control	Cultured cells grown in prescence of BrdU or tissues from experimental animals injected with BrdU.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	0.N.35
Host	Mouse
Concentration	~0.2mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See B2850-07X.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Bromodeoxyuridine (BrdU) conjugated to BSA. Cellular Localization: Nuclear
Specificity	Recognizes Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU conjugated to a protein carrier, as well as free BrdU. Species Crossreactivity: all species
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.