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You are here:Home » Antibodies » Abs to Nucleotides » Anti -Bromodeoxyuridine (BrdU)

Anti -Bromodeoxyuridine (BrdU)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC IF
BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Catalog #B2850-07
Applications
Suitable for use in Flow Cytometry, Immuno uorescence and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry (formalin/paraf n): 1:500 for 32 minutes at RT. For staining of formalin- xed tissues, incubate sections in 4N HCl for 30 minutes at RT, followed by digestion with trypsin at 1mg/ml PBS, 10 minutes at 37ºC .
Optimal dilutions to be determined by the researcher.
Positive ControlCultured cells grown in prescence of BrdU or tissues from experimental animals injected with BrdU.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.35
HostMouse
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See B2850-07X.
PurityPurified by Protein G affinity chromatography.
ImmunogenBromodeoxyuridine (BrdU) conjugated to BSA. Cellular Localization: Nuclear
SpecificityRecognizes Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU conjugated to a protein carrier, as well as free BrdU. Species Crossreactivity: all species
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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