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You are here:Home » Antibodies » Abs to Nucleotides » Anti -Bromodeoxyuridine (BrdU) (BSA & Azide Free)

Anti -Bromodeoxyuridine (BrdU) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC IF
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Catalog #B2850-07X
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry(frozen & formalin/paraf n):1ug/ml for 30 minutes at RT.
For staining of formalin- xed tissues, incubate sections in 4N HCl for 30 minutes at RT, followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37ºC
Optimal dilutions to be determined by the researcher.
Positive ControlCultured cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.
Cellular LocalizationNuclear
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.35
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4
Also available with BSA and azide. See B2850-07.
PurityPurified by Protein G affinity chromatography.
ImmunogenBromodeoxyuridine (BrdU) labeled with BSA.
SpecificityRecognizes free BrdU, BrdU coupled to protein carrier, BrdU in ssDNA (partially denatured dsDNA). Species Reactivity: All species
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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