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You are here:Home » Antibodies » Abs to Nucleotides » Anti -Bromodeoxyuridine (BrdU) (Biotin)

Anti -Bromodeoxyuridine (BrdU) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified FC
Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Catalog #B2850-10
ApplicationsSuitable for use in Flow Cytometry. Other applications have not been tested.
Recommended Dilution:
Flow Cytometry: 1:20
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q392
HostMouse
Concentration~250ug/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
PurityPurified immunoglobulin
ImmunogenIodouridine conjugated to ovalbumin.
SpecificityRecognizes BrdU in ssDNA, free BrdU or BrdU coupled to a protein carrier. Crossreactivity: iodouridine. Does not react with thymidine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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