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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IH FC

Bromodoxyuridine (BrdU) can be incorporated into DNA during S-phase of the cell cycle. The antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labelling techniques.
Catalog #	B2850-10G
Applications	Suitable for use in Flow Cytometry and Immunohistochemistry (frozen, paraffin). Other applications not tested.
Recommended Dilution	Flow Cytometry: 1:25-1:100; 10ul labels 10e6 cells in 100ul
	Optimal dilutions to be determined by the researcher.
Hybridoma	NS1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	10B320
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Bromodeoxyuridine (BSA).
Specificity	Recognizes the thymidine analog bromodoxyuridine (BrdU).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	BUdr