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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IH

BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Catalog #	B2850-24B
Applications	Immunohistology (Formalin/paraffin): (Use Ab at 1ug/ml for 30 min at RT) [For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37°C]
Positive Control	Cultured cells grown in presence of BrdU or tissues from experimental animals
	injected with BrdU.
Cellular Localization	Nuclear
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5G15
Host	Mouse
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.
Purity	Purified by Protein G affinity chromatography.
Immunogen	BrdU conjugated to BSA.
Specificity	Reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. Species Crossreactivity: All species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.