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You are here:Home » Antibodies » Abs to Nucleotides » Anti -Bromodeoxyuridine (BrdU)

Anti -Bromodeoxyuridine (BrdU)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant IH
5-bromodeoxyuridine (BrdU) is an analogue of thymi- dine. When incubated with tissue, proliferating cells incorporate BrdU into the DNA during S phase and this provides an accurate means of determining cell prolifer- ation. This antibody detects incorporated BrdU in cells or tissues.
Catalog #B2850-24C
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:200-1:400 for 1 hr. at RT
Optimal dilutions to be determined by the researcher.
Positive Control
Cells or tissues with incorporated Brdu, nuclear staining pattern
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No85-2C8
HostMouse
ConcentrationNot determined
FormSupplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
PuritySupernatant
ImmunogenConjugate of BrdU and helix pomatia haemocyanin.
SpecificityRecognizes Bromodeoxyuridine. Species Crossreactivity: Any species with incorporated BrdU.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names5-Bromo-2'-deoxyuridine


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