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Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant IH

5-bromodeoxyuridine (BrdU) is an analogue of thymi- dine. When incubated with tissue, proliferating cells incorporate BrdU into the DNA during S phase and this provides an accurate means of determining cell prolifer- ation. This antibody detects incorporated BrdU in cells or tissues.
Catalog #	B2850-24C
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 1:200-1:400 for 1 hr. at RT
	Optimal dilutions to be determined by the researcher.
Positive Control
	Cells or tissues with incorporated Brdu, nuclear staining pattern
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	85-2C8
Host	Mouse
Concentration	Not determined
Form	Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
Purity	Supernatant
Immunogen	Conjugate of BrdU and helix pomatia haemocyanin.
Specificity	Recognizes Bromodeoxyuridine. Species Crossreactivity: Any species with incorporated BrdU.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	5-Bromo-2'-deoxyuridine