| In the past, most cell-proliferation studies have used radioactive thymidine as an incorporated label to give evidence of DNA replication. Recently, a less hazardous technique has been developed using bromodeoxyuridine (BrdU), a thymidine analog, in lieu of a radioactive reagent. (1) BrdU is incorporated into proliferating cells (S-phase) much in the same way as radioactive thymidine, but is then detected by a monoclonal anti-BrdU antibody, and revealed using a highly sensitive streptavidin- biotin staining system. BrdU has proven useful for proliferative studies of normal and neoplastic tissues both in vivo and in vitro. |
|
| Catalog # | B2851 |
| The BrdU staining system uses a biotinylated monoclonal anti-BrdU, thus eliminating the need for a species-specific secondary antibody. As a result, BrdU labeling can be performed in rats and mice without problems of crossreactivity or spurious staining. |
| Streptavidin-peroxidase is used as a signal generator for the BrdU system. Diaminobenzidine (DAB) in the presence of hydrogen peroxide is used as a chromogen, staining BrdU-incorporated nuclei dark brown. |
| This kit also contains four BrdU control slides for the convenience of the investigator | four unstained positive controls. |
| Kit Components | B2851-01 Trypsin Concentrate 1x3ml |
| B2851-02 Trypsin Diluent 1x12ml |
| B2851-03 Denaturing Solution 1x6ml (Ready-to-use) |
| B2851-04 Blocking Solution 1x6ml(Ready-to-use) |
| B2851-05 BrdU (Biotin) 1x6ml (Ready-to-use) |
| B2851-06 Streptavidin (HRP) 1x6ml (Ready-to-use) |
| B2851-07 Substrate Buffer, 20x 1x2ml |
| B2851-08 DAB, 20x 1x2ml |
| B2851-09 0.6% Hydrogen Peroxide, 20x 1x2ml |
| B2851-10 Hematoxylin 1x6ml (Ready-to-use) |
| B2851-11 Histomount™ 1x6ml (Ready-to-use) |
| B2851-12 Control Slides 4x Unstained Controls |
| Materials Required but not Provided | -Phosphate Buffered Saline (PBS) |
| -Quenching solution for endogenous peroxidase |
| -Alcohol and xylene |
| -Cover slips |
| Storage and Stability | Store at 4°C. Stable for at least 6 months. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
