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You are here:Home » Antibodies » Abs to Mitosis Proteins » Anti -BRD4 (Bromodomain-containing Protein 4, Protein HUNK1, HUNK1)

Anti -BRD4 (Bromodomain-containing Protein 4, Protein HUNK1, HUNK1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Brd4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; longer variant has a C-terminus region with a proline and glutamine-rich domains. Brf4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. Brd4 acts at different stages of cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. Brf4 functions as a cellular target for E2 protein of papillomavirus.
Catalog #B2852-83B
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 2-5ug/ml
Optimal dilutions to be determined by the researcher.
Positve ControlHeLa cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity Chromatography.
ImmunogenSynthetic peptide corresponding to aa150-200 of human Brd4 (NP_490597).
SpecificityRecognizes human Brd4. BRD4 is expressed in two forms, the long form is approximately 1400 amino acids and the short form is approximately 722 amino acids.
Species CrossreactivityChimpanzee, mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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