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Specifications

5'-G C A A T G N N^-3'
3'-C G T T A C^N N-5'
Catalog #	B2910
Concentration	5u/ul
Source	Bacillus stearothermophilus Isl 15–111
Buffer	10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 55°C. (Incubation at 37°C results in 20% activity.)
Diluent Buffer	10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage Buffer	10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion Assay	No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BseMI.
Ligation/Recutting Assay	After 50-fold overdigestion (3u/ug DNA x 17 hours) with BseMI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.6uM. Approximately 80% of these can be recut.
Labeled Oligonucleotide (LO) Assay	No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Stability during Prolonged Incubation	A minimum of 0.3units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 55°C.
Thermal Inactivation	Enzyme is inactivated by incubation at 80°C for 20min.
Number of Recognition Sites in DNA
Lambda	44
PhiX174	4
M13mp18/19	3
pBR322	2
pUC18/19	2
pUC57	2
pTZ19R/U	2
pBluescriptIIKS(-/+)	2
pBluescriptIISK(-/+)	2
pACYC177	3
pACYC184	1
Unit Definition	One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 55°C in 50ul of assay buffer.