Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

5'-C T C A G (N)10^-3'
3'-G A G T C (N)8 ^-5'
Catalog #	B2910-05
Concentration	1u/ul
Source	Bacillus stearothermophilus Isl 15–111
[Buffer] + SAM	[33mM Tris-acetate (pH 7.9), 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA] + 0.01mM S-adenosylmethionine (supplied with 0.5mM solution). Incubate at 55°C. (Incubation at 37°C results in 30% activity.)
Diluent Buffer	10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage Buffer	10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion Assay	No detectable change in the specific fragmentation pattern is observed after 64-fold overdigestion (4u/ug lambda DNA x 16 hours) with BseMII.
Ligation/Recutting Assay	After 10-fold overdigestion (2u/ug DNA x 5 hours) with BseMII more than 90% of the DNA fragments can be ligated at a 5'-termini concentration of 0.5uM. None of these can be recut due to the methylation at the recognition sequence by restriction enzyme.
Labeled Oligonucleotide (LO) Assay	No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Stability during Prolonged Incubation	A minimum of 0.5units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 55°C.
Thermal Inactivation	Enzyme is inactivated by incubation at 80°C for 20min.
Number of Recognition Sites in DNA
Lambda	79
PhiX174	10
M13mp18/19	23
pBR322	7
pUC18/19	5
pUC57	5
pTZ19R/U	4
pBluescriptIIKS(-/+)	4
pBluescriptIISK(-/+)	4
pACYC177	13
pACYC184	8
Unit Definition	One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 55°C in 50ul of assay buffer.