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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » BseMII

BseMII

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Specifications

5'-C T C A G (N)10^-3'
3'-G A G T C (N)8 ^-5'
Catalog #B2910-05
Concentration 1u/ul
Source Bacillus stearothermophilus Isl 15111
[Buffer] + SAM [33mM Tris-acetate (pH 7.9), 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA] + 0.01mM S-adenosylmethionine (supplied with 0.5mM solution). Incubate at 55°C. (Incubation at 37°C results in 30% activity.)
Diluent Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after 64-fold overdigestion (4u/ug lambda DNA x 16 hours) with BseMII.
Ligation/Recutting AssayAfter 10-fold overdigestion (2u/ug DNA x 5 hours) with BseMII more than 90% of the DNA fragments can be ligated at a 5'-termini concentration of 0.5uM. None of these can be recut due to the methylation at the recognition sequence by restriction enzyme.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Stability during Prolonged IncubationA minimum of 0.5units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 55°C.
Thermal InactivationEnzyme is inactivated by incubation at 80°C for 20min.
Number of Recognition Sites in DNA
Lambda79
PhiX17410
M13mp18/1923
pBR3227
pUC18/195
pUC575
pTZ19R/U4
pBluescriptIIKS(-/+)4
pBluescriptIISK(-/+)4
pACYC17713
pACYC1848
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 55°C in 50ul of assay buffer.


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