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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » Bsh1285I (McrI)

Bsh1285I (McrI)

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Specifications

5'-C G Pu Py^C G-3'
3'-G C^Py Pu G C-5'
Catalog #B2922-05
Concentration 10u/ul
Source Bacillus sphaericus RFL1285
Buffer 10mM Tris-HCl (pH 7.5), 10mM MgCl2, 50mM NaCl and 0.1mg/ml BSA. Incubate at 37°C
Diluent Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol, or Storage Buffer.
Storage Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 150mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Bsh1285I.
Ligation/Recutting AssayAfter 50-fold overdigestion (3u/ug DNA x 17 hours) with Bsh1285I, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.1uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Methylation EffectBlocked by CG methylation.
Stability during Prolonged IncubationA minimum of 0.2units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Thermal InactivationEnzyme is inactivated by incubation at 65°C for 20min.
Digestion of Agarose-embedded DNAA minimum of 5units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Compatible EndsCGGC^CG–BseNI, Cfr42I CGGC^TG–BseNI CGAT^CG–PacI, PvuI, SgfI
Number of Recognition Sites in DNA
Lambda22
PhiX1741
M13mp18/194
pBR3227
pUC18/195
pUC575
pTZ19R/U5
pBluescriptIIKS(-/+)6
pBluescriptIISK(-/+)6
pACYC1776
pACYC1846
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.


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