Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Molecular Biology » MB-Enzymes, Restriction » BshTI (AgeI)

BshTI (AgeI)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

5'-A^C C G G T-3'
3'-T G G C C^A-5'
Catalog #B2930
Concentration 10u/ul
Source Bacillus sphaericus Jo 22024
Buffer 50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA. Incubate at 37°C
Diluent Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with BshTI (see Star Activity).
Ligation/Recutting AssayAfter 50-fold overdigestion (3u/ug DNA x 17 hours) with BshTI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.13uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Blue/White Cloning AssayThe mix of pUC57/HindIII, pUC57/PstI and pUC57/Eco32I digests was incubated with 10units of enzyme for 1 hour. After religation and transformation 0.4% of white colonies were detected.
Star ActivityA large excess of enzyme (10u/ug DNA x 16 hours) may result in star activity.
Methylation EffectsBshTI does not cut ACm5CGGT. Blocked by CG methylation.
Stability during Prolonged IncubationA minimum of 0.1units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Thermal InactivationEnzyme is inactivated by incubation at 65°C for 20min.
Digestion of Agarose-embedded DNAA minimum of 20units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Compatible EndsCfr10I, Kpn2I, Cfr9I, Eco88I (C^CCGGG), BsaWI, SgrAI.
Number of Recognition Sites in DNA
Lambda13
PhiX1740
M13mp18/190
pBR3220
pUC18/190
pUC570
pTZ19R/U0
pBluescriptIIKS(-/+)0
pBluescriptIISK(-/+)0
pACYC1772
pACYC1844
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.


External Links