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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » Bsp120I (ApaI)

Bsp120I (ApaI)

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Specifications

5'-G^G G C C C-3'
3'-C C C G G^G-5'
Catalog #B2945
Unlike ApaI, Bsp120I produces DNA fragments with a 4-base 5'-extension.
Concentration 10u/ul, 50u/ul(HC)
Source Bacillus subtilis RFL120
Buffer 10mM Tris-HCl (pH 7.5), 10mM MgCl2 and 0.1mg/ml BSA. Incubate at 37°C.
Diluent Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Storage Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after 240-fold overdigestion (15u/ug lambda DNA x 16 hours) with Bsp120I.
Ligation/Recutting AssayAfter 50-fold overdigestion (3u/ug DNA x 17 hours) with Bsp120I, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.01uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Blue/White Cloning AssayPUC57 was digested at a unique site with 10units of enzyme for 16 hours. After religation and transformation 0.6% of white colonies were detected.
Methylation EffectsBsp120I does not cut GGGCCm5C. Blocked by overlapping Dcm or CG methylation.
Stability during Prolonged IncubationA minimum of 0.1units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Thermal InactivationEnzyme is inactivated by incubation at 80°C for 20min.
Digestion of Agarose-embedded DNAA minimum of 5units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Compatible EndsCfrI, Eco52I, NotI
Number of Recognition Sites in DNA
Lambda1
PhiX1740
M13mp18/190
pBR3220
pUC18/190
pUC571
pTZ19R/U0
pBluescriptIIKS(-/+)1
pBluescriptIISK(-/+)1
pACYC1770
pACYC1840
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1ug of lambda DNA-CpoI fragments in 1 hour at 37°C in 50ul of assay buffer.


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