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You are here:Home » Molecular Biology » MB-Protein Kinases » Btk, Recombinant, Human, NT, GST-His-Tag (Brutonís Tyrosine Kinase)

Btk, Recombinant, Human, NT, GST-His-Tag (Brutonís Tyrosine Kinase)

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Specifications

Human BTK (GenBank Accession No. NM_000061), full length with N-terminal GST tag, MW= 106kD, expressed in an Baculovirus infected Sf9 cell expression system.
Catalog #B5000-02
ApplicationSuitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Specific Activity~20 pmol/min/ug
Assay ConditionsEnzyme reaction was carried out for 1 hour at RT in a buffer containing 50mM HEPES, pH 7.5, 10mM MgCl2, 1mM EDTA, 0.01% BRIJ-35, 200uM ATP
Substrate: 2uM Tyr peptide 1.
Storage and StabilityAliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Molecular Weight106kD
SourceBaculovirus infected Sf9
Purity~ 80% by SDS-PAGE
FormSupplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween-20, 3 mM DTT, 50% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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