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You are here:Home » Molecular Biology » MB-Protein Kinases » Btk, active, Recombinant, Human (Brutonís Tyrosine Kinase)

Btk, active, Recombinant, Human (Brutonís Tyrosine Kinase)

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Specifications

N-terminal His6-tagged, recombinant, full-length, human BTK, expressed by baculovirus in Sf21 insect cells.
Catalog #B5001
Specific Activity ~200U/mg
Unit DefinitionOne unit of BTK activity is defined as 1nmol phosphate incorporated into 250uM S6504 Src, Substrate Peptide (KVEKIGEGTYGVVYK) per minute at 30°C with a final ATP concentration of 100uM.
MS Tryptic FingerprintConfirmed identity as BTK with 50% amino acid coverage of the translated
sequence.
BTK Kinase Assay2.5-25ng phosphorylates 250uM S6504 Src, Substrate Peptide, in the assay described in Protocols..
Storage and StabilityAliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Handling Recommendations
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot
unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
SourceSf21
Purity~50-60% by SDS PAGE and Coomassie blue staining. MW 78.4kD.
Concentration~0.1mg/ml
FormSupplied as a frozen liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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