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Anti -BTLA (B and T Lymphocyte Attenuator, CD272)
Anti -BTLA (B and T Lymphocyte Attenuator, CD272)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
E B |
|
| B and T Lymphocyte Attenuator (BTLA) also known as CD272, is a 70kD type I transmembrane glycoprotein of the CD28 family of T cell costimulatory molecules. BTLA functions as a coinhibitor on activated T cells and on B cells and dendritic cells. The extracellular domain (ECD) of this Ig superfamily protein contains one V-type Ig-like domain, which interacts with the herpesvirus entry mediator (HVEM/TNFSF14). The amino acid sequence of the ECD of human BTLA shares only 34% identity to that of mouse BTLA. One human BTLA variant lacking the transmembrane sequence has been reported. | | | Catalog # | B5001-60 | | Applications | Suitable for use in Western Blot and Direct ELISA. Other applications not tested. | | Recommended Dilutions | Western Blot: 1-2ug/ml. Using a colormetric detection system, the detection limit is ~5ng/lane under non-reducing and reducing conditions. | | Direct ELISA: 0.5-1ug/ml. The detection limit is ~2ng/well. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 7H92 | | Host | Rat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ull sterile PBS. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | NS0-derived recombinant human BTLA (aa25-150) Accession # Q7Z6A9 | | Specificity | Recognizes human BTLA. Does not crossreact with recombinant mouse BTLA or recombinant human HVEM. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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