| Human CD272 (BTLA, B and T Lymphocyte Attenuator) is a member of the Immunoglobulin superfamily and has sequence homology to PD-1 and CTLA4. It is expressed on T and B lymphocytes and other hemopoetic lineages. Engagement of this molecule by its ligand, CD270 (HVEM, a TNF superfamily member) can down regulate activated T and B cell responses. CD272 levels on antigen specific CD8+ T cells have been reported to decrease in viral specific, but not melanoma specific activated lines. Polymorphism of the CD272 molecule has been linked to rheumatoid arthritis. |
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| Catalog # | B5001-61K |
| Applications | Suitable for use in ELISA and Flow Cytometry. Other applications not tested. |
| Recommended Dilutions | ELISA: Detection |
| Flow Cytometry: 1:50 |
| Optimal dilutions to be determined by the researcher. |
| Flow Cytometry Protocol | 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells (PBMC) per tube were pelleted and pre incubated 5 minutes with 20ul of human IgG at 300ug/ml, after which they were washed and incubated 45 minutes on ice with 80ul of B5001-61K at a 1:50 dilution (5ug/ml). |
| 2. They were then washed three times, fixed and analyzed by FACS using a lymphoid gate. |
| 3. Cells stained positive with a mean shift of 0.54 log10 fluorescent units when compared to a Mouse IgG1 (FITC) negative control at a similar concentration. |
| 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml of B5001-61G. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 11K3 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.25mg/ml |
| Form | Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC). |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Recombinant protein corresponding to soluble human CD272 |
| Specificity | Recognizes human CD272 (BTLA). Binds to CD272 on the cell surface of human PBMC in Flow Cytometry and to recombinant CD272-muIg in ELISA. Does not block binding of Biotinylated CD270 (HVEM)-muIg to CD272-muIg in ELISA, but appears to enhance this interaction. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | B- and T-lymphocyte Associated |
