Suitable for use in FLISA and Flow Cytometry. Other applications not tested.

Flow Cytometry: 1:50 (10ug/ml) Optimal dilutions to be determined by the researcher.

1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells (PBMC) per tube were pelleted and pre incubated 5 minutes with 20ul of human IgG at 300ug/ml, after which they were washed and incubated 45 minutes on ice with 80ul of B5001-61L diluted 1:50 (10ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS using a lymphoid gate. 3. Cells stained positive with a mean shift of 1.15 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD272 antibody (Catalog #B5001-61G).

May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Recombinant soluble human CD272.

Supplied as a liquid in 50mM sodium phosphate pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2 BSA, 15% glycerol, 0.04% sodium azide. Labeled with R-Phycoerythrin (PE).

Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).

Recognizes human BTLA. Binds to CD272 on the cell surface of human PBMC in Flow cytometry and to recombinant CD272-muIg (B5001-61M) in EIA. Does not block binding of Biotinylated CD270(HVEM)-muIg (H2033-98A) to CD272-muIg in EIA, but appears to enhance this interaction.