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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E

Catalog #	B8997-10
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions	Optimal dilutions to be determined by the researcher.
Specificity	Using a conjugate 1,3-Butadiene, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
	Compound Crossreactivity ratio(a)Butadiene-BSA 1 Acrolein-BSA 1/20,000Formaldehyde-BSA 1/>50,000Malondialdehyde-BSA 1/>50,000 Butanoic acid-BSA 1/>50,000Propionic acid-BSA 1/>50,000
(a)	1,3-Butadiene-BSA concentration/conjugated close structure concnetration at half displacement.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 2 months at 4°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	Not Determined
Form	Supplied as a lyophilized powder. Reconstitute with 100ul sterile dH2O.
Purity	Purified by protein carrier (BSA) affinity column, and ammonium sulphate precipitation.
Immunogen	Synthetic 1,3-Butadiene conjugated to BSA.
Specificity	Recognizes 1,3-Butadiene conjugated with BSA. See additional crossreactivity data.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.