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You are here:Home » Antibodies » Antibodies-Complement » Anti -Complement C1q, Human

Anti -Complement C1q, Human


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Clone Host Grade Applications
Monoclonal Mouse Supernatant E B
C1q is serum glycoprotein of 18-polypeptides chains consisting of three non-identical subunits, A (29kD), B (26kD) and C (246aa, 19kD) in molar ratio of 1:2:2. Detection of C1q in serum, in serum-C1 and within immune complexes; inhibits hemolytic activity of C1 by binding to C1q in the liquid phase (antibody concentration necessary to inhibit 50% of hemolytic activity in the fluid phase is ~0.85ug/ml). With C1 bound to immune complexes a dose dependent increase of C1 activity was observed at antibody concentrations of 1.5ug/ml. Useful for detection of C1q-bearing immune complexes in patients with rheumatic disorders.
Catalog #C0010-06
ApplicationsSuitable for use in ELISA, Western Blot and Inhibition experiments (e.g. hemolytic activity). Other applications not tested.
Recommended DilutionWestern Blot: 1:10-1:50 (ECL method). Incubate for 1hour at RT.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No5F91
ConcentrationNot determined
FormSupplied as a liquid.
PurityHybridoma culture supernatant
ImmunogenPurified human complement C1q.
SpecificityRecognizes human Complement C1q. Polypeptide Reacting: C-chain of complement C1q (25kD in SDS-PAGE).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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