| The complement component C1 is a protein complex that consists of six subunits of C1q.Each C1q subunit of the complex is known to bind to immune complexes, which often are deposited in basement membrane.C1q is a versatile recognition protein that triggers activation of the classical pathway of complement, where the first step involves the binding of the globular |
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| domain of Clq to the Fc regions of aggregated IgG or IgM.The C1q globular domain is a heterotrimer of the C-terminal halves of one A, one B, and one C chain, held together |
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| mainly by non-polar interactions, with a Ca2+ ion bound at the top.A modular organization has been suggested of the globular domain, consistent with the view that A, B, and C |
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| chains are functionally autonomous modules and have distinct and differential ligand-binding properties (1-3). |
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| Applications: |
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| Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
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| Catalog # | C0010-10T |
| Recommended Dilution | Flow Cytometry: 1:50 |
| Western Blot: 1:500-1:1000 |
| Immunoprecipitation: 1:10 |
| Immunohistochemistry: 1:100-1:250 |
| Immunocytochemistry: 1:250-1:500 |
| Optimal dilutions to be determined by the researcher. |
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| Storage and Stability: |
| May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 9H301 |
| Host | Rabbit |
| Source | Human |
| Concentration | As reported |
| Form | Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA. |
| Purity | Supernatant |
| Immunogen | A synthetic peptide corresponding to residues in human C1q. |
| Specificity | Recognizes human C1q. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
