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C2C12 Nuclear Extract, Differentiated (Muscle, Myoblast)
C2C12 Nuclear Extract, Differentiated (Muscle, Myoblast)
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| Permanent murine cell lines of fast and slow skeletal myocyte derivation. | | | Catalog # | C0010-13 | | Positive Transcription Factors | Western Blot: | | Myf-4, Sp1, Sp3, TFIIB | | EMSA | E12/E47, Myf-3 (MyoD), Sp1 | | Storage and Stability | For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. | | Purity | Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). | | Concentration | ~5mg/ml | | Form | Supplied as a liquid in 20mM Hepes, pH 7.9, 100mM KCl, 1mM MgCl2, 20% glycerol, 0.5mM PMSF, 0.5mM DTT. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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