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You are here:Home » Antibodies » Abs to Peptides » Anti -Complement C3d (C3d, Complement C3d fragment)

Anti -Complement C3d (C3d, Complement C3d fragment)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Purified
Catalog #C0010-16A
ApplicationsSuitable for use in DID, RID and Rocket IEP. Other applications not tested.
Recommended DilutionRID and Rocket IEP: 7ul antiserum/cm2 in gel vs. 5ul fresh plasma
Double Immunodiffusion: 10ul antiserum vs. 5ul plasma
Note: The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Numbern/a
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~23ng/ml
FormSupplied as a liquid in glycine buffered saline, pH7.4, 0.1% EACA, 0.01% nenzamidine,1mM EDTA, 0.1% sodium azide
PurityPurified
ImmunogenHuman C3d purified from pooled activated serum, shown to be pure by IEP. Cellular Localization: Secreted.
SpecificityRecognizes human complement C3d. Gives a single arc in the alpha region when tested by IEP against aged human plasma (C3d) and a continuous double arc in the alpha and beta regions when tested against fresh plasma (C3 and C3d). Identity has been confirmed by double diffusion (Ouchterlony) vs human plasma and a known antihumanC3d. Does not crossreact with rabbit, rat, horse, mouse, bovine, canine, cat, guinea pig or chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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